Phylogeny, resistome and mobile genetic elements of emergent OXA-48 and OXA-245Klebsiella pneumoniaeclones circulating in Spain

Noncarbapenemase OXA-48 Variants (OXA-163 and OXA-405) Falsely Detected as Carbapenemases by the β Carba Test.

Laurent Dortet, Thierry Naas Associated French National Reference Center for Antibiotic Resistance, Le Kremlin-Bicêtre, France; Research Unit EA7361 “Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases,” Faculty of Medicine, University Paris-Sud, Le Kremlin-Bicêtre, France; Department of Bacteriology-Parasitology-Hygiene, Bicêtre Hospital, Assistance Publique—Hôpitaux de ...

OXA-48-like carbapenemases: the phantom menace.

OXA-48-type carbapenem-hydrolysing class D β-lactamases are increasingly reported in enterobacterial species. To date, six OXA-48-like variants have been identified, with OXA-48 being the most widespread. They differ by a few amino acid substitutions or deletions (one to five amino acids). The enzymes hydrolyse penicillins at a high level and carbapenems at a low level, sparing broad-spectrum c...

Comparative evaluation of a novel chromogenic medium (chromID OXA-48) for detection of OXA-48 producing Enterobacteriaceae.

Comparative evaluation of the recently developed chromogenic culture medium chromID OXA-48 (bioMérieux) with chromID CARBA (bioMérieux) and SUPERCARBA showed that chromID OXA-48 and SUPERCARBA media have the highest sensitivity for detection of OXA-48 producing Enterobacteriaceae (91% and 93%) comparatively to chromID CARBA (21 %). The chromID OXA-48 has the highest specificity, with 100%, as c...

Evaluation of a new phenotypic OXA-48 disk test for differentiation of OXA-48 carbapenemase-producing Enterobacteriaceae clinical isolates.

The current phenotypic methods for detecting carbapenemase-producing Enterobacteriaceae (CPE) allow differentiation between class A and B carbapenemases, but they cannot confirm in a single test class D OXA-48 carbapenemase producers. In this study, we evaluated a new phenotypic test, the OXA-48 disk test, which is based on an imipenem disk and two blank disks adjacent to the imipenem disk, loa...

Emergence of OXA-48-producing Enterobacteriaceae in Switzerland.